Protein adsorption to planar electrochemical sensors and sensor materials
C. Lim, S. Slack, S. Ufer, and E. Lindner
Joint Graduate Program in Biomedical Engineering,
Department of Biomedical
Engineering, The University of Memphis, Memphis, TN 38152, USA; Premitec
Inc., Venture II, Suite 101, 920 Main Campus Drive, Raleigh, NC 27606,
Abstract: In electrochemical sensing devices, aimed for acute
and chronic in vivo application, the active surface of the sensor is
often negligible compared to the overall surface area of the device
in contact with the biological host. Consequently, to minimize the perturbation
of an implanted sensor on the in vivo environment the chemical composition
and surface texturing of the complete device (the active sensor, sensor
substrate, and accessories) have to be considered. In our
work, the adsorption of three abundant proteins (albumin, IgG, and fibrinogen)
was determined quantitatively on untreated and modified sensor substrates
and sensing membrane surfaces. In this study, a flexible polyimide-based
material (Kapton ®) was used as sensor substrate with or without
an amorphous diamond-like carbon (DLC) or an amorphous oxygen-containing
DLC (o-DLC) coating. The ion-sensitive membranes were cast from
high-molecular-weight (HMW) or carboxylated poly(vinyl chloride) (PVC)
and were doped with increasing concentrations of highly hydrophilic
poly(ethylene oxide) (PEO). The potentiometric characteristics of the
potassium-selective membranes cast with up to 6 % PEO were the same
as those without PEO. However, the PEO-modified PVC membranes elicited
a large amount of protein adsorption, especially in terms of albumin.
*Plenary lectures presented at the Inaugural Conference for the Southern and Eastern Africa Network of Analytical Chemists (SEANAC), Gaborone, Botswana, 7-10 July 2003. Other presentations are published in this issue, pp. 697-888.
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