Temporal responses to estrogen in the uterus
K. C. Fertuck and T. R. Zacharewski
Biochemistry and Molecular Biology, National Food
Safety and Toxicology Center, and Institute for Environmental Toxicology,
Michigan State University, East Lansing, MI 48824-1319, USA
Abstract: The estrogen receptor acts primarily as an estrogen-inducible
transcription factor in target organs, however, until recently the identification
of individual responsive transcripts has been cumbersome. In the present
study, oligonucleotide GeneChip microarrays were used to describe and
analyze the levels of approximately 6500 transcripts in the uteri of
immature, ovariectomized mice at various times following oral exposure
to 100 mg/kg of ethynyl estradiol. The most
reproducible responses were identified and subjected to K-means clustering,
and functional annotations were obtained for the transcripts within
each cluster. Connections were made between the observed transcriptional
responses and the known physiological responses to estrogen exposure,
focusing here on effects on uterine cell cycle progression leading to
estrogen-stimulated uterine growth.
*Report from a SCOPE/IUPAC project: Implication of
Endocrine Active Substances for Human and Wildlife (J. Miyamoto and
J.Burger, editors). Other reports are published in this issue,
pp. 1617-2615.
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