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Pure Appl. Chem., Vol. 71, No. 2, pp.349-359, 1999

CHEMISTRY AND THE ENVIRONMENT DIVISION

COMMISSION ON OILS, FATS AND DERIVATIVES

The determination of stigmastadienes in vegetable oils. Results of collaborative studies and the standardized method

(Technical report)

M.C. Dobarganes, A. Cert and A. Dieffenbacher

Abstract: The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the steroidal hydrocarbons is then analysed by capillary gas chromatography and the quantitative assessment made by the use of an internal standard /cholea-3,5-staddiene). The procedure is very sensitive allowing determination at levels of 0.01 - 4.0 mg/kg.

Full Text (144k pdf)


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