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Pure Appl. Chem. 76(4), 829-837, 2004
Pure and Applied Chemistry
Vol. 76, Issue 4
Proteomics principles and challenges
Department of Analytical Chemistry, Lund University,
Box 124, SE-221 00 Lund, Sweden
Abstract: Proteomics studies rely on high-resolving separation
techniques whereby the biological complexity can be unraveled. Both
gel-based and liquid-phase separation principles are needed in order
to mine deeper into the low abundant area of the proteins expressed
in human cell samples. The expression levels that can be reached today
with proteomics technology are still only reaching part of all proteins
present in complex biological samples. The challenge to be met is not
an easy task and will, in many aspects, be determined by the speed and
success whereby the analytical field can make milestone progress. Examples
will be given from our research group on proteomics studies, as well
as principles and challenges
within this research area.
*Plenary lectures presented at the Inaugural Conference for the Southern and Eastern Africa Network of Analytical Chemists (SEANAC), Gaborone, Botswana, 7-10 July 2003. Other presentations are published in this issue, pp. 697-888.
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