Title: Electrochemical DNA-based biosensors: terms and methodology
Chairman: Jan Labuda
Members: Ana Maria Oliveira Brett, Gennady Evtugyn, Miroslav
Fojta, Marco Mascini, Mehmet Ozsoz, Emil Palecek, and Joseph Wang
To bring in terms of definitions or definition-like characterization
and classification of DNA electrochemistry and DNA-based sensors as
well as related methodology and to provide analytical, electrochemical,
biomedical and environmental communities with critical evaluation on
In 1999, the IUPAC technical report "Electrochemical
biosensors: recommended definitions and classification" was
published [PAC 71(12), 2333-2348, 1999].
This report did not deal extensively with DNA as the biological recognition
element but considered the work on other types of biosensors in future.
Since that time a significant progress in the development and application
of electrochemical sensors based on DNA and other nucleic acids (including
aptamers and peptide nucleic acids) has been achieved. On the other
hand, so far there have been no efforts to essential classification
in this dynamically developing field. Through this project it is proposed
to achieve the critical evaluation of the terms and methodology related
to electrochemical DNA sensors.
The project deals with:
- a) DNA-based biocomponents (natural, biomimetic) including their
- b) type of interaction to be addressed (DNA hybridization, DNA-drug,
interactions, aptamer-antigen, etc.,
- c) detection principles (label-free, label-based, reagentless, indicator-based
- d) construction of sensors and DNA chips,
- f) specific performance criteria.
In each part, the classification of terms and methodology will be accompanied
with a short introducing state description and illustrating schemes.
As a result, the project will evaluate critically the terms and methodology
in the field. General and applicable nomenclature of the IUPAC technical
report from 1999 will be accepted and referred.
One special workshop will be organized within the project close to
mid-term meeting to discuss and further develop the classification and
evaluation DNA-based sensors. Hence, the technical report should be
valuable used as a key instruction for wide audience from academic,
biomedical, environmental and food-testing, drug-developing etc. labs
to sensors producers.
Last Update: 4 September 2007
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